Title: LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome

Authors (18): K. -Y. Lai, S. R. G. .Galan, Y. Zeng, T. H. Zhou, C. He, R. Raj, J. Riedl, S. Liu, K. P. Chooi, N. Garg, M. Zeng, L. H. Jones, G. J. Hutchings, S. Mohammed, S. K. Nair, J. Chen, B. G. Davis, W. A. van der Donk

Themes: New Catalysts (2021), Featured (2021)

DOI: 10.1016/j.cell.2021.04.001

Citations: 40

Pub type: article-journal

Publisher: Elsevier BV

Issue: 10

License: [{"URL"=>"https://www.elsevier.com/tdm/userlicense/1.0/", "start"=>{"date-parts"=>[[2021, 5, 1]], "date-time"=>"2021-05-01T00:00:00Z", "timestamp"=>1619827200000}, "delay-in-days"=>0, "content-version"=>"tdm"}]

Publication date(s): 2021/05 (online)

Pages: 2680-2695.e26

Volume: 184 Issue: 10

Journal: Cell

Link: [{"URL"=>"https://api.elsevier.com/content/article/PII:S0092867421004360?httpAccept=text/xml", "content-type"=>"text/xml", "content-version"=>"vor", "intended-application"=>"text-mining"}, {"URL"=>"https://api.elsevier.com/content/article/PII:S0092867421004360?httpAccept=text/plain", "content-type"=>"text/plain", "content-version"=>"vor", "intended-application"=>"text-mining"}]

URL: http://dx.doi.org/10.1016/j.cell.2021.04.001

Name Description Publised
LanCLs have C-glutathionylation activity with potential to trap the eliminylome Enzyme-mediated damage repair or containment, whilst common for nucleic ... 2020
LanCL Mass spectrometry and Western blot data supporting the function of LanCL... 2021
Eukaryotic LanCL2 protein Eukaryotic LanCL2 protein... 2021
Table S1. Unbiased protein pull-down and proteomics analysis, related to Figure 1E. Table S1. Unbiased protein pull-down and proteomics analysis, related to... 2021
Table S2. Chemical generation of Dha-containing MEK1 variants and their kinase activity, related to Figures 3, 4, S2, S3, S4, S5, and S6. Table S2. Chemical generation of Dha-containing MEK1 variants and their ... 2021
Table S3. Analysis of the proteomic literature for the presence of Dha and Dhb proteins and their conjugates with GSH, β-mercaptoethanol, Cys, and dithiothreitol, related to Figures 5 and S7. Table S3. Analysis of the proteomic literature for the presence of Dha a... 2021
Table S4. Oligonucleotide sequences used in this study, related to the STAR Methods. Table S4. Oligonucleotide sequences used in this study, related to the S... 2021
Methods S1. NMR spectra of bisalkylating agents used to introduce dehydro amino acids into proteins, related to Figures 3 and 4. Methods S1. NMR spectra of bisalkylating agents used to introduce dehydr... 2021


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